条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Genes Cells, 9, 549-560 (2004).<br>寄託後、2年間については、使用した研究の成果発表に際しては共著とする。それ以降は謝辞の表明を必要とする。特許等の申請、および事業活動を行う場合は、寄託者と別途協議を行う。 Heterozygote x Wild-type [C57BL/6JJcl] <a href='https://brc.riken.jp/mus/pcr02712'>Genotyping protocol -PCR-</a> TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] ・戻し交配バックグラウンド系統：C57BL/6N（日本クレア）・ホモで胎生期に死亡 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells, 9, 549-560 (2004).The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using the BIOLOGICAL RESOURCE for two years after deposition to the RIKEN BRC. After two years from deposition, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. true 秋山　徹 Developmental Biology Research Gpr129 knock-out mice. Gpr129, G-protein-coupled receptor 126 is a member of seven-transmembrane proteins related to family-B G-protein- coupled receptors (LNB-TM7), expresses at high levels in the heart and somites during mouse embryogenesis and in the adult lung. Homozygous mutant mice are embryonic lethal (E12.5). RBRC02712 ES 細胞 TT2 に homologous recombination により lacZ-neo を挿入。ICR マウス胚盤胞に注入し，できたキメラを C57BL/6N とかけあわせた。 C（3〜6か月） Developed by Dr. Akiyama T, The University of Tokyo. ・戻し交配 バックグラウンド系統：C57BL/6N（日本クレア） ・ホモで胎生期に死亡 C (3-6 months) E. coli neo, E. coli lacZ, polyoma enhancer/herpes simplex virus thymidine kinase promoter (HSV tk promoter)(MC1), Phage P1 loxP sites Tetsu AKIYAMA B6.Cg-Gpr126<tm1Taki> B6.Cg-Gpr126<tm1Taki> DREG Knock-out Mouse DREG Knock-out Mouse Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.iqb.u-tokyo.ac.jp/5ken/" target="_blank">Lab HP (Japanese)</A>